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pluripotent nt2 d1 embryonal carcinoma cells  (ATCC)


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    ATCC pluripotent nt2 d1 embryonal carcinoma cells
    Pluripotent Nt2 D1 Embryonal Carcinoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 806 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nt2+cells/NTERA-2+cl%2ED1/pm42252325-169-15-20
    Average 96 stars, based on 806 article reviews
    pluripotent nt2 d1 embryonal carcinoma cells - by Bioz Stars, 2026-10
    96/100 stars

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    Labeling:

    Article Title: Low level of plasma DNase is associated with worse clinical outcome in testicular germ cell tumor patients and exogeneous DNase I improves (Leverages) cisplatin treatment efficacy
    Article Snippet: Cell lines NCCIT (ATCC CRL2073), NOY-1 (ENG101, Kerafast, Japan) and SuSa (ACC747, DSMZ, Braunschweig, Germany) were maintained in RPMI-1640 medium (Sigma-Aldrich) containing 10% fetal bovine serum (FBS, GIBCO TM ), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAX TM -I Supplement (GIBCO TM ). .. NTERA-2 (ATCC CRL1973) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC HTB-106, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC CRL-7002) were cultivated in high‐glucose (4.5 g/L) DMEM (Sigma-Aldrich) supplemented with 10% FBS (GIBCO TM ), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAX TM -I Supplement (GIBCO TM ). ..

    Article Title: Low level of plasma DNase is associated with worse clinical outcome in testicular germ cell tumor patients and exogeneous DNase I improves (Leverages) cisplatin treatment efficacy.
    Article Snippet: Cell lines NCCIT (ATCC® CRL2073TM), NOY-1 (ENG101, Kerafast, Japan) and SuSa (ACC747, DSMZ, Braunschweig, Germany) were maintained in RPMI-1640 medium (Sigma-Aldrich®) containing 10% fetal bovine serum (FBS, GIBCOTM), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAXTM-I Supplement (GIBCOTM). .. NTERA-2 (ATCC® CRL1973TM) labeled NT2 cells throughout the manuscript, Tera-2 cells (ATCC® HTB-106TM, kindly provided by Dr. Ludmila Boublikova, Charles University and University Hospital in Motol, Prague, the Czech Republic) and testicular fibroblasts Hs 1.Tes (ATCC® CRL-7002TM) were cultivated in high‐glucose (4.5 g/L) DMEM (Sigma-Aldrich®) supplemented with 10% FBS (GIBCOTM), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAXTM-I Supplement (GIBCOTM). .. Cell lines NCCIT (ATCC CRL2073), NOY-1 (ENG101, Kerafast, Japan) and SuSa (ACC747, DSMZ, Braunschweig, Germany) were maintained in RPMI-1640 medium (Sigma-Aldrich) containing 10% fetal bovine serum (FBS, GIBCOTM), 10,000 IU/mL penicillin (Biotika, Slovakia), 5 μg/mL streptomycin and 1x GlutaMAXTM-I Supplement (GIBCOTM).

    Cell Culture:

    Article Title: New feature of hMEIOB and hSPATA22 binding to ssDNA from a single-molecule perspective
    Article Snippet: The 3D structure of ssDNA with 12 bases was randomly generated via Avogardro 2, and the molecular docking was performed by AutoDock, and the bonding relationship between ssDNA and SPATA22 truncates was demonstrated by PyMoL. .. HEK-293T cells and NT2 cells were purchased from American Type Culture Collection (ATCC; Manassas, USA) and cultured in DMEM (HyClone, Logan, USA) supplemented with 10% fetal bovine serum (FBS) and 100 U/mL penicillin and streptomycin in a humidified 5% CO 2 atmosphere. .. The vectors were constructed and extracted with an EndoFree Mini Plasmid Kit II (TIANGEN, Beijing, China).

    Article Title: New feature of hMEIOB and hSPATA22 binding to ssDNA from a single-molecule perspective.
    Article Snippet: The 3D structure of ssDNA with 12 bases was randomly generated via Avogardro 2, and the molecular docking was performed by AutoDock, and the bonding relationship between ssDNA and SPATA22 truncates was demonstrated by PyMoL. .. Cell culture and transfection HEK-293T cells and NT2 cells were purchased from American Type Culture Collection (ATCC, Manassas, USA) and cultured in DMEM (HyClone, Logan, USA) supplemented with 10% fetal bovine serum (FBS) and 100 U/ml penicillin and streptomycin in a humidified 5% CO2 atmosphere. .. The vectors were constructed and extracted with an EndoFree Mini Plasmid Kit II (TIANGEN, Beijing, China).

    Modification:

    Article Title: Comparative analysis of lipid-peptide nanoparticles prepared via microfluidics, reverse phase evaporation, and ouzo techniques for efficient plasmid DNA delivery.
    Article Snippet: In the current “era of lipid carriers,” numerous strategies have been developed to manufacture lipid nanoparticles (LNPs).. Nevertheless, the potential impact of various preparation methods on the characteristics, use, and/or stability of these LNPs remains unclear.. In this work, we attempted to compare the effects of three different preparation methods: microfluidics (MF), reverse phase evaporation (RV), and ouzo (OZ) on lipid-peptide NPs (LPNPs) as plasmid DNA delivery carriers.

    Transfection:

    Article Title: New feature of hMEIOB and hSPATA22 binding to ssDNA from a single-molecule perspective.
    Article Snippet: The 3D structure of ssDNA with 12 bases was randomly generated via Avogardro 2, and the molecular docking was performed by AutoDock, and the bonding relationship between ssDNA and SPATA22 truncates was demonstrated by PyMoL. .. Cell culture and transfection HEK-293T cells and NT2 cells were purchased from American Type Culture Collection (ATCC, Manassas, USA) and cultured in DMEM (HyClone, Logan, USA) supplemented with 10% fetal bovine serum (FBS) and 100 U/ml penicillin and streptomycin in a humidified 5% CO2 atmosphere. .. The vectors were constructed and extracted with an EndoFree Mini Plasmid Kit II (TIANGEN, Beijing, China).



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    Image Search Results


    ( A )YAP1 protein is expressed in different cell types relevant to the brain, such as astrocytes (CCF-STTG1), microglia (HMC3), neuroblastoma (SH-SY5Y), and NT2 cells, as demonstrated by immunoblotting. Data are mean ± SEM ( n = 3). ( B ) Images show immunocytochemical evidence of YAP1 expression (in green) and DAPI-stained nuclei (in blue) in different cell types and human neurons. Scale: 20 µm.

    Journal: Neurology International

    Article Title: YAP1 Upregulates Cytoskeleton Regulator ARHGEF1 and Tissue Regeneration Factor NEDD9 in a Multiplex Proteomic Study

    doi: 10.3390/neurolint18050096

    Figure Lengend Snippet: ( A )YAP1 protein is expressed in different cell types relevant to the brain, such as astrocytes (CCF-STTG1), microglia (HMC3), neuroblastoma (SH-SY5Y), and NT2 cells, as demonstrated by immunoblotting. Data are mean ± SEM ( n = 3). ( B ) Images show immunocytochemical evidence of YAP1 expression (in green) and DAPI-stained nuclei (in blue) in different cell types and human neurons. Scale: 20 µm.

    Article Snippet: We purchased astrocytoma (cat# CCF-STTG1), HMC3 (cat # CRL-3304), SH-SY5Y (cat # CRL2266), and Ntera-2 (NT2) cells (cat # CRL-1973) from ATCC (Manassas, VA, USA) as cellular models of astrocytes, microglia, neuroblastoma, and neurons, respectively.

    Techniques: Western Blot, Expressing, Staining